副猪嗜血杆菌lgtF基因缺失株的构建及其部分生物学特性

doi:10.11843/j.issn.0366-6964.2015.11.019

Abstract

Abstract:

The lgtF encodes a glucosyltransferase responsible for adding a glucose to get heptose I in the synthesis of Lipooligosaccharide （LOS）.The lgtF gene is an important virulence-associated gene in some Gram-negative bacteria.However，the function of lgtF gene in Haemophilus parasuis was still unknown.We constructed an ΔlgtF mutant （ΔlgtF） of H.parasuis SC096 using a natural transformation system.The LOS of H.parasuis SC096 strain，ΔlgtF mutant was extracted by the hot phenol-water method.The LOS was addressed by SDS-PAGE and sliver stain.The growth curve，Serum resistance activity，adhesion and invasion ability in porcine kidney epithelial cells （PK-15） and porcine umbilical vein endothelial cells （PUVEC） of wild type SC096 and ΔlgtF mutant were measured.Compared to the wild-type SC096 strain，the ΔlgtF mutant exhibited obvious truncation of LOS structure and slight growth defect.Furthermore，the mutant had a greater sensitivity to the bactericidal action of porcine and rabbit serum （P<0.05），and displayed a significantly reduced ability to adhere to and invade PK-15 and PUVEC （P<0.05）.The above findings suggested that the lgtF gene is a virulence-associated gene of the H.parasuis SC096 strain.

CLC Number:

S852.613

ZENG Ze，HE Huan，YUE Hua，TANG Cheng，ZHANG Bin. Construction and Characterization of a Haemophilus parasuis SC096 ΔlgtF Mutant Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 2056-2062.

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Construction and Characterization of a Haemophilus parasuis SC096 ΔlgtF Mutant Strain

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